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High Speed FRET based SNP Genotyping Measurement on the PHERAstar
BMG LABTECH, KBiosciences
Single Nucleotide Polymorphisms (SNP`s) have become an invaluable tool in the field of Genetic Research. Here we show the use of BMG LABTECH´s PHERAstar multimode HTS plate reader for high speed FRET based SNP Genotyping measurement. The new KASPar™ system, developed by KBiosciences, was used to assess the best performance of the PHERAstar. Optimized optical modules with dual emission lead to improvements in speed and accuracy of measurement.
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Molecular Probes® NanoOrange® Assay Performed on BMG LABTECH FLUOstar OPTIMA Microplate Reader
BMG LABTECH
The fluorescent NanoOrange® Protein Quantitation Kit from Molecular Probes® (Invitrogen) can be easily adapted for use with a microplate reader such as the FLUOstar OPTIMA from BMG LABTECH and used in a high throughput manner. High and small protein concentration range is evaluated. NanoOrange® can also be performed on other BMG LABTECH microplate readers, including the FLUOstar and POLARstar Omegas, as well as the NOVOstar and PHERAstar.
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SUMO FRET-based assays performed on BMG LABTECH’s NOVOstar
BMG LABTECH
The small ubiquitin-like modifiers SUMO1 and SUMO2 and their processing by the protease SenP1 was investigated by using an in vitro FRET assay performed on BMG LABTECH´s NOVOstar. The described assay has applications in SUMO protease characterization, enzyme kinetic analysis and high-throughput inhibitor screening.
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Investigation of the stereoselectivity of an anti-amino acid antibody utilizing tryptophan fluorescence
BMG LABTECH
The binding sites of proteins such as antibodies are known to often
contain tryptophan (Trp) residues, whose fluorescent properties may be
altered upon ligand binding. Conformational changes within the binding
site or simply the presence of the ligand can result in either fluorescence
quenching or enhancement, which may be utilized to quantitatively
investigate protein-ligand interactions.
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A New Matrix Application Device for MALDI Tissue Imaging
Bruker Daltonics
ImagePrep™ provides highly reproducible matrix preparations for MALDI tissue imaging in a fully automated process.
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Cisbio´s HTRF® Cortisol Assay
BMG LABTECH and Cisbio International
Cortisol is a corticosteroid hormone present in many metabolic processes, inducing key enzymes of the whole metabolism. Cisbio´s HTRF® cortisol assay allows fast and efficient determination of cortisol in complex samples such as serum and whole cells. In this poster we describe the performance of the cortisol assay on BMG LABTECH´s PHERAstar. With its optimized HTRF® optical module the PHERAstar is the ideal tool to run HTRF® assays.
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Analysis of Prostate Tumour Cell Invasion Using BD FluoroBlok and FLUOstar OPTIMA
BMG LABTECH and Paterson Institute for Cancer Research, University of Manchester
The FLUOstar OPTIMA is used for reading the BD Biosciences FluoroBlok™ tumour cell invasion system. In this poster we show that the results are comparable to the traditional invasion assays. The introduced method is fast, saving time and labour. The FLUOstar OPTIMA is able to take multiple readings over time at a user defined temperature.
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HTRF® IP-One Assay Performed on the PHERAstar and RUBYstar Plate Readers
BMG LABTECH and Cisbio International
BMG LABTECH is a leader in microplate reader technology. Cisbio’s HTRF IP-ONE screening assay for the Gq coupled GPCR pathway has been applied on two of BMG LABTECH´s HTS readers. One reader is the RUBYstar, a dedicated time-resolved fluorescence (TRF) HTS machine; the other is the PHERAstar, a multifunctional HTS machine that can read in all detection modes (fluorescence intensity, TRF, fluorescence polarization, luminescence, and absorbance).
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Invitrogen’s LanthaScreen™ TR-FRET Tyrosine Kinase Assay Using the PHERAstar
Invitrogen Corporation and BMG LABTECH
Several tyrosine kinases were characterized using Invitrogen´s LanthaScreen™ assay. The LanthaScreen™ TR-FRET platform combines terbium as the donor group and fluorescein as the acceptor species.BMG LABTECH’s PHERAstar microplate reader provides the ideal platform to simplify assay development. With its dual wavelength emission detection and five photomultiplier tubes, the PHERAstar provides the speed and sensitivity needed to take full advantage of Invitrogen’s LanthaScreen™ technology.
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Invitrogen’s LanthaScreen™ TR-FRET Protein Kinase C Assay Using the PHERAstar
Invitrogen Corporation and BMG LABTECH
BMG LABTECH’s PHERAstar microplate reader provides the ideal platform to simplify assay development. With its dual wavelength emission detection and five photomultiplier tubes, the PHERAstar provides the speed and sensitivity needed to take full advantage of Invitrogen’s LanthaScreen™ technology.
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Promega’s Multiplexed Cell Viability and Apoptosis Assays Performed on the PHERAstar
Promega Corporation and BMG LABTECH
Today’s high-throughput screening facilities face increasing demands to generate more information from their existing compound libraries. In this application note, we demonstrate the combination of several Promega cell-based assays (CellTiter-Blue®, Apo-One® and Caspase-Glo® 3/7) multiplexed in both low-volume 384 and 1536-well plate formats. The BMG LABTECH PHERAstar microplate reader is used to record both luminescence and fluorescence, depending on the multiplex combination.
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Promega’s Multiplexed Luciferase Reporter and Cell Viability Assays performed on the PHERAstar
BMG LABTECH and Promega Corporation
By multiplexing a reporter assay (EnduRen™, ViviRen™) with a cell viability assay (CellTiter-Glo®), it is possible to determine if reporter response variations are due to changes in cell number and health. In this poster, we demonstrate the combination of several Promega cell-based assays multiplexed in both low-volume 384- and 1536-well plate formats using the BMG LABTECH PHERAstar microplate reader to record luminescence.
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Molecular Probes PicoGreen® Assay Performed on BMG LABTECH POLARstar OPTIMA Microplate Reader
BMG LABTECH
The PicoGreen® dsDNA quantitation reagent from Molecular Probes is a highly sensitive fluorescence assay for dsDNA detection. Using BMG LABTECH´s POLARstar OPTIMA for measuring this quantitation assay a linear range of more than four magnitudes is obtained. The limit of detection was calculated to be 21 pg dsDNA / 200 µl showing that the POLARstar OPTIMA meets all requirements needed for a high performance.
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Simultaneous Dual Emission Detection for Fast Kinetic BRET Assays
BMG LABTECH
Bioluminescence Resonance Energy Transfer (BRET) is a system for monitoring intermolecular interactions in vivo. The BRET2™ demo kit has been used to prove the feasibility of performing a BRET assay on the POLARstar OPTIMA microplate reader. The POLARstar OPTIMA’s internal reagent injectors for 384-well plate format combined with high-end simultaneous dual emission detection offer a unique advantage for fast kinetic assays where simultaneous emission detection at two wavelengths is required.
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ORAC Assay on the FLUOstar OPTIMA to Determine Antioxidant Capacity
BMG LABTECH
BMG LABTECH has performed and optimized the fluorescence-based Oxygen Radical Absorbance Capacity (ORAC) assay on the multifunctional FLUOstar Optima microplate reader. The ORAC assay can be measured both directly and indirectly. The FLUOstar Optima has the ability to read in three modes (absorbance, luminescence, and fluorescence), has a standard 45°C incubation chamber, and has optional onboard injectors; thus making it the ideal machine to perform the ORAC assay.
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Detection of Tyrosine Kinase Activity using the PHERAstar in AlphaScreen™ mode
BMG Labtech
Tyrosine kinases are important regulators in cellular processes. Kinases have been found to be involved in diseases such as cancer and cardiovascular, therefore, molecules that modulate kinase function are expected to be promising new drug targets. In this application note, we describe the performance of an AlphaScreen™ tyrosine kinase assay on BMG LABTECH´s PHERAstar using an AlphaScreen™ specific excitation LASER.
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Assay Optimization and Profiling in uHTS Format
Deerac Fluidics,Promega Corp
We demonstrate the use of Promega’s Kinase-Glo® Plus Luminescent Kinase Assay for miniaturized kinase assays using Deerac Fluidics’ Equator™ HTS liquid handling system. The Kinase-Glo® Plus Assay is a homogeneous method of measuring kinase activity by quantifying ATP in solution following a kinase reaction. The Equator™ HTS is a noncontact liquid dispenser capable of delivering volumes from 20µl down to 50nl.
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Keeping DMSO Concentration Below 0.5% to Minimize its Effect in HTS Assays
Deerac Fluidics,Labcyte
The Labcyte Echo™ 550 liquid handler utilizes acoustic drop ejection (ADE) to transfer 2.5-250 nL of compounds in DMSO directly from storage plates to assay plates. Deerac Fluidics Latitude™ bulk dispenser, which uses “spot-on” technology, can precisely deliver as low as 50 nL. The Latitude can be used to rapidly add pure DMSO to specific wells so that all assay wells have the same DMSO concentration.
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Automated Dispensation of Yttrium Oxide SPA Imaging Beads, into 1536-Well Plates Using Deerac Fluidics’ Equator™ HTS Pipetting System
Deerac Fluidics
Here we demonstrate the successful combination of the use of the Equator HTS pipetting system to dispense Streptavidin coupled Yttrium Oxide (YOx) SPA Imaging beads. 2ul of YOx beads were dispensed into1536-well plates containing an excess of 3H-biotin. Plates were read on LEADseeker™ Multimodality Imaging System and analyzed for accuracy and reproducibility. CVs of 6-7% were obtained. Outliers were within spec of 10 or less per 1000 wells.
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